Low-cost, simple, and scalable self-assembly of DNA origami nanostructures

摘要: Despite demonstrating exciting potential for applications such as drug delivery and biosensing, the development of nanodevices practical broader use in research education are still hindered by time, effort, cost associated with DNA origami fabrication. Simple robust methods to perform scale self-assembly process critical facilitate translation industrial or clinical applications. We report a simple approach fold nanostructures that is fast, robust, scalable. demonstrate fabrication at scales approximately 100–1,500-fold higher than typical scales. further an we termed low-cost efficient annealing (LEAN) involving initial heating 65 °C 10 min, then 51 2 h, followed brief quenching 4 leads effective assembly range structures tested. In contrast other scaling assembly, this can be carried out using cheap widely available equipment (e.g., hot plates, water baths, laboratory burners) uses standard recipes materials so readily applied any existing new designs. envision these device commercial education.