Expression of basement membrane type IV collagen and type IV collagenases (MMP-2 and MMP-9) in human fetal teeth

摘要: Formation and degradation of dental basement membrane (BM) are important for tooth development. Data on the expression genes type IV collagen (the major structural component BM) collagenases [MMP-2 (72 kDa) MMP-9 (92 kDa)], enzymes that degrade during human development, lacking. We studied MMP-2 in fetal teeth (from 13th to 20th gestational weeks, covering cap stage through early hard tissue formation). During bell stages, situ hybridization located transcripts alpha 1 chain fibroblasts surrounding enamel organ. No mRNA was detected germ epithelium or papilla. However, immunoreactivity observed BM underlying up appositional stage. Transcripts were mostly cells papilla follicle. Transient inner late cap/early bell-stage teeth. apposition, a high level confined secretory odontoblasts. by sensitive reverse-transcription polymerase reaction (RT-PCR) developing Thus, BM, may be mesenchymal cell origin. Further, but not participate remodeling morphogenesis.