Oxidative folding of reduced and denatured huwentoxin-I.

作者: Songping Liang , Qin Shu , Xianchun Wang , Xiang Zong

DOI: 10.1023/A:1020693920990

关键词: ThiolDenaturation (biochemistry)PeptideCystineCysteineBiochemistryChemistryOxidative foldingOxidative phosphorylationGlutathioneChromatography

摘要: … The difference in absorbance at 412 nm between the toxin … ) containing 0.5 M guanidine hydrochloride at a concentration … In the analysis of the oxidative folding, the HPLC column was …

参考文章(11)
G A Jamieson, M Hasitz, T Okumura, Platelet glycocalicin. Interaction with thrombin and role as thrombin receptor of the platelet surface. Journal of Biological Chemistry. ,vol. 253, pp. 3435- 3443 ,(1978) , 10.1016/S0021-9258(17)34819-6
Youxing Qu, Songping Liang, Jizhen Ding, Xiuchai Liu, Renji Zhang, Xiaocheng Gu, Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution. Journal of Protein Chemistry. ,vol. 16, pp. 565- 574 ,(1997) , 10.1023/A:1026314722607
Thomas E. Creighton, Protein structure : a practical approach IRL Press at Oxford University Press. ,(1990)
AK Ahmed, SW Schaffer, DB Wetlaufer, Nonenzymic reactivation of reduced bovine pancreatic ribonuclease by air oxidation and by glutathione oxidoreduction buffers. Journal of Biological Chemistry. ,vol. 250, pp. 8477- 8482 ,(1975) , 10.1016/S0021-9258(19)40784-9
Paul K. Pallaghy, Raymond S. Norton, Katherine J. Nielsen, David J. Craik, A common structural motif incorporating a cystine knot and a triple-stranded beta-sheet in toxic and inhibitory polypeptides. Protein Science. ,vol. 3, pp. 1833- 1839 ,(1994) , 10.1002/PRO.5560031022
Paul K. Pallaghy, Brendan M. Duggan, Michael W. Pennington, Raymond S. Norton, Three-dimensional Structure in Solution of the Calcium Channel Blocker ω-Conotoxin Journal of Molecular Biology. ,vol. 234, pp. 405- 420 ,(1993) , 10.1006/JMBI.1993.1595
Dongyi Zhang, Songping Liang, Assignment of the three disulfide bridges of huwentoxin-I, a neurotoxin from the spider selenocosmia huwena. Journal of Protein Chemistry. ,vol. 12, pp. 735- 740 ,(1993) , 10.1007/BF01024931
W.L. Anderson, D.B. Wetlaufer, A new method for disulfide analysis of peptides Analytical Biochemistry. ,vol. 67, pp. 493- 502 ,(1975) , 10.1016/0003-2697(75)90323-1
Tad A. Holak, J. Habazettl, H. Oschkinat, J. Otlewski, Structures of proteins in solution derived from homonuclear three-dimensional NOE-NOE nuclear magnetic resonance spectroscopy. High-resolution structure of squash trypsin inhibitor Journal of the American Chemical Society. ,vol. 113, pp. 3196- 3198 ,(1991) , 10.1021/JA00008A072
Youxing Qu, Songping Liang, Jizhen Ding, Libin Ma, Renji Zhang, Xiaocheng Gu, Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 1. Sequence-specific 1H-NMR assignments. Journal of Protein Chemistry. ,vol. 14, pp. 549- 557 ,(1995) , 10.1007/BF01886881