Electrophoretic Study of the Interaction of Radioactive Poliovirus with Components of Cultured Cells.

摘要: Thorne, H. V. (University of Minnesota, Minneapolis). Electrophoretic study the interaction radioactive poliovirus with components cultured cells. J. Bacteriol. 85: 1247–1255. 1963.—The P 32 -labeled postnuclear fraction HeLa cell homogenates was first examined using radioactivity to quantitate reaction. The effects virus and debris concentration, suspending medium, temperature, time on reaction were determined. Binding independent salt concentration up 1.0 m unaffected by calcium magnesium ions at 10 −3 m. particulate mammalian cells in culture sucrose gradient zone electrophoresis pH 7.2 a simple apparatus which permitted several simultaneous analyses. distributions for different types distinct appeared be influenced conditions culture. Addition homogenate before analysis used identify virus-binding activity. Activity human esophageal epithelium found mainly membranous fractions relatively low electrophoretic mobility. Components broader spread mobility moderately active amnion, but uncultured amnion had almost undetectable activity distribution components. Rat heart L did not bind poliovirus, binding present both ERK-1 CRE rabbit lines.