Fluorimetric determination of pyridoxal in dried milk

作者: Vlasta Fiedlerová , Jiří Davídek

DOI: 10.1007/BF01810484

关键词: LactoneChromatographyFluorescenceChemistryPyridoxalReaction conditionsChromatographic separationBiotechnologyFood scienceIndustrial and Manufacturing EngineeringBiochemistryGeneral chemistry

摘要: The determination of pyridoxal, pyridoxol and pyridoxamin was studied by means the fluorimetric method measuring fluorescence lactone 4-pyridoxic acid being formed from pyridoxal KCN in weakly alkaline solutions; under given reaction conditions is specific sensitive. Chromatographic separation on Dowex 50W × 8 column makes it possible to determine even more complicated model solutions (in presence milk proteins) powdered milk.

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