High IFN-γ production by CD8+ T cells and early sensitization among infants at high risk of atopy

摘要: Abstract Background High genetic risk (HR) of atopy among unstratified populations infants is associated with attenuated IFN-γ responses. However, the role in progression from HR status to active disease less clear. Objective To identify immune function markers neonates that are positive atopic outcomes at 2 years. Methods Cord blood mononuclear cells (CBMCs) were collected 175 children and cryopreserved. The assessed for by skin prick 0.5 CBMCs thawed stimulated allergens mitogens PHA staphylococcal enterotoxin B (SEB), cytokine responses determined. Results No correlations observed between allergen-specific CBMC outcomes. In contrast, sensitization was strongly polyclonal both ( P =.002) SEB =.005), also SEB-induced IL-5 =.05), IL-10 =.02), IL-13 =.01). Logistic regression analysis identified elevated PHA-induced as strongest independent predictors development. Cell separation studies confirmed CD8 + T source ∼90% production. Conclusions produced may synergize H cytokines driving development HR.