Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.

摘要: Membrane proteins (such as ion channels, transporters, and receptors) secreted are essential for cellular activities. N-linked glycosylation is involved in stability function of these occurs at Asn residues. In several organs, profiles N-glycans have been determined by comprehensive analyses. Nevertheless, the cochlea mammalian inner ear, a tiny organ mediating hearing, has yet to be examined. Here, we focused on stria vascularis, an epithelial-like tissue cochlea, characterised liquid chromatography with mass spectrometry. This hypervascular not only expresses transporters channels control electrochemical balance but also harbours different receptors that maintain structure activity organ. Seventy-nine glycans were identified rat vascularis. Among these, 55 glycans, complete structures determined; other 24 species, partial glycosidic linkage patterns full monosaccharide composition identified. process characterisation, sialylated subjected sequentially two alkylamidation reactions; this derivatisation helped distinguish α2,3-linkage α2,6-linkage sialyl isomers These data should accelerate elucidation molecular architecture cochlea.