Filtering technique for fluorescence-based DNA sequencing data

摘要: An important aspect in data processing for the four dye fluorescence-based DNA sequencing is crosstalk filtering. Typically, a matrix M, which function on fluorophores employed and fluorescence detection system, used multicomponent analysis. In deconvolution process applied directly to raw signal, linear assumption. This necessitates signal be aligned baseline. The various techniques aligning have as negative effect an additional distortion of signal. algorithm removal presented. It based using variation (instead itself) thus making possible before base line adjustment. addition, supplementary filtering step proposed order account fact that reality nonlinear. second T accounts influence each signals from derivatives other three. overall result less information lost through Our strategy helps preserving contained data. Consequently, we consider processed our can allow better accuracy calling.