Simple Light-Triggered Fluorescent Labeling of Silica Nanoparticles for Cellular Imaging Applications.
作者： Rui Sun , Ling Yin , Shaohua Zhang , Lei He , Xiaju Cheng
关键词: Chemistry 、 Covalent bond 、 Fluorescein 、 Internalization 、 Nanotechnology 、 Surface modification 、 Nanomaterials 、 Diazirine 、 Fluorescence 、 Cytotoxicity
摘要: A simple and mild light-triggered fluorescent labeling approach, based on the photolysis reactive carbene insertion of diazirine, to rapidly generate nanoparticles is reported. Proof concept studies demonstrated that non-fluorescent SiNPs could be covalently labeled by diazirine-conjugated fluorescein (dFITC) upon photo irradiation (λ=365 nm) afford with good stability low cytotoxicity. In vitro cellular imaging results indicated internalization into living cells readily visualized fluorescein-labeled (F-SiNPs) in real time. We believe approach may offer a valuable method for surface modification nanomaterials achieve new functions.
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