Layered double hydroxide nanoparticles as cellular delivery vectors of supercoiled plasmid DNA
作者： Tara L Walker , G Q Max Lu , Zhi Ping Xu , Kerh-lin Liu , Helen M Cooper
DOI: 10.2147/IJN.S
关键词: Biology 、 Kidney metabolism 、 Transfection 、 FuGENE 、 Gene delivery 、 DNA 、 Base pair 、 Viability assay 、 Molecular biology 、 Plasmid
摘要: We prepared stable homogeneous suspensions with layered double hydroxide (LDH) nanoparticles for in vitro gene delivery tests. The viability of HEK 293T cells the presence LDH at different concentrations was investigated. This revealed 50% cell 500 microg/mL that is much higher than 50-100 used supercoiled pEF-eGFP plasmid (ca. 6100 base pairs) mixed nanoparticle anion exchange a weight ratio DNA/LDH between 1:25 and 1:100. In experiments show GFP expression starts first day, reaches maximum levels by second day continues third day. generally increases increase DNA loading DNA-LDH nanohybrids. However, efficiency as agent low. For example, relative 7%-15% commercial FuGENE 6. Three to 6% total expressed an amount detectable FACS cytometry 2 days after transfection 1 25 nanomaterial. lower could be attributed aggregation caused long-chain DNA.
uq.edu.au参考文章(18)
Zhi Ping Xu, Gregory S. Stevenson, Chao-Qing Lu, Gao Qing (Max) Lu, Perry F. Bartlett, Peter P. Gray, Stable suspension of layered double hydroxide nanoparticles in aqueous solution. Journal of the American Chemical Society. ,vol. 128, pp. 36- 37 ,(2006) , 10.1021/JA056652A
J. F. Marko, E. D. Siggia, Statistical mechanics of supercoiled DNA Physical Review E. ,vol. 52, pp. 2912- 2938 ,(1995) , 10.1103/PHYSREVE.52.2912
Léa Desigaux, Malha Ben Belkacem, Peggy Richard, Joël Cellier, Philippe Léone, Laurent Cario, Fabrice Leroux, Christine Taviot-Guého, Bruno Pitard, None, Self-Assembly and Characterization of Layered Double Hydroxide/DNA Hybrids Nano Letters. ,vol. 6, pp. 199- 204 ,(2006) , 10.1021/NL052020A
Gunnar P.H. Dietz, Mathias Bähr, Delivery of bioactive molecules into the cell: the Trojan horse approach Molecular and Cellular Neuroscience. ,vol. 27, pp. 85- 131 ,(2004) , 10.1016/J.MCN.2004.03.005
Joon Sig Choi, Kihoon Nam, Jong-yeun Park, Jung-Bin Kim, Ja-Kyeong Lee, Jong-sang Park, Enhanced transfection efficiency of PAMAM dendrimer by surface modification with L-arginine. Journal of Controlled Release. ,vol. 99, pp. 445- 456 ,(2004) , 10.1016/J.JCONREL.2004.07.027
H. Cai, A. C. Hillier, K. R. Franklin, C. C. Nunn, M. D. Ward, Nanoscale imaging of molecular adsorption Science. ,vol. 266, pp. 1551- 1555 ,(1994) , 10.1126/SCIENCE.266.5190.1551
Stephanie Rhaese, Hagen von Briesen, Helga Rübsamen-Waigmann, Jörg Kreuter, Klaus Langer, Human serum albumin-polyethylenimine nanoparticles for gene delivery. Journal of Controlled Release. ,vol. 92, pp. 199- 208 ,(2003) , 10.1016/S0168-3659(03)00302-X
David J. Chen, Brian S. Majors, Alexander Zelikin, David Putnam, Structure–function relationships of gene delivery vectors in a limited polycation library Journal of Controlled Release. ,vol. 103, pp. 273- 283 ,(2005) , 10.1016/J.JCONREL.2004.11.028
Colin W Pouton, Leonard W Seymour, Key issues in non-viral gene delivery Advanced Drug Delivery Reviews. ,vol. 34, pp. 3- 19 ,(1998) , 10.1016/S0169-409X(98)00048-9
P E Nielsen, Systemic delivery: the last hurdle? Gene Therapy. ,vol. 12, pp. 956- 957 ,(2005) , 10.1038/SJ.GT.3302507