作者: Carlos Garbisu , Olatz Garaiyurrebaso , Lur Epelde , Elisabeth Grohmann , Itziar Alkorta
关键词: Biology 、 Microorganism 、 Bioaugmentation 、 Bacteria 、 Soil contamination 、 Horizontal gene transfer 、 Microbiology 、 Organic matter 、 Bioremediation 、 Plasmid
摘要: Bioaugmentation, or the inoculation of microorganisms (e.g., bacteria harboring required catabolic genes) into soil to enhance rate contaminant degradation, has great potential for bioremediation soils contaminated with organic compounds. Regrettably, cell bioaugmentation frequently turns an unsuccessful initiative, owing rapid decrease bacterial viability and abundance after inoculation, as well limited dispersal inoculated in matrix. Genes that encode degradation compounds are often located on plasmids and, consequently, they can be spread by horizontal gene transfer well-established, ecologically competitive, indigenous populations. Plasmid-mediated aims stimulate genes among introduction plasmids, donor cells, such genes. But acquisition recipient cells affect host's fitness, a crucial aspect success plasmid-mediated bioaugmentation. Besides, environmental factors moisture, temperature, matter content) play important roles efficiency expression horizontally acquired finally, activity. For reproducible, much more research is needed better selection strains accompanying together in-depth understanding populations conditions plasmid functioning interest.