Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise
作者： Kelvin Tsai , Tai-Ger Hsu , Kuang-Ming Hsu , Hu Cheng , Tsung-Yun Liu
DOI: 10.1016/S0891-5849(01)00729-8
关键词: Gel electrophoresis 、 DNA 、 Peripheral blood mononuclear cell 、 Aerobic exercise 、 Reactive oxygen species 、 Lipid peroxidation 、 Endocrinology 、 Endonuclease 、 Molecular biology 、 Comet assay 、 Internal medicine 、 Chemistry 、 Physiology (medical) 、 Biochemistry
摘要: Abstract Reactive oxygen species produced during vigorous exercise may permeate into cell nuclei and induce oxidative DNA damage, but the supporting evidence is still lacking. By using a 42 km marathon race as model of massive aerobic exercise, we demonstrated significant degree unrepaired base oxidation in peripheral immunocompetent cells, despite concurrent increase urinary excretion 8-hydroxy-2′-deoxyguanosine. Single gel electrophoresis with incorporation lesion-specific endonucleases further revealed that oxidized pyrimidines (endonuclease III-sensitive sites) contributed to most postexercise nucleotide oxidation. The damage correlated significantly plasma levels creatinine kinase lipid peroxidation metabolites, lasted for more than 1 week following race. This phenomenon be one mechanisms behind immune dysfunctions after exhaustive exercise.
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sci-hub.se 参考文章(41)
Henning B. Nielsen, Birgitte Hanel, Steffen Loft, Henrik E. Poulsen, Bente K. Pedersen, Marcus Diamant, Kim Vistisen, Niels H. Secher, Restricted pulmonary diffusion capacity after exercise is not an ARDS-like injury. Journal of Sports Sciences. ,vol. 13, pp. 109- 113 ,(1995) , 10.1080/02640419508732218
Carol A Delaney, Irene C Green, Jillian E Lowe, James M Cunningham, Anthony R Butler, Louise Renton, Ieta D'Costa, Michael H.L Green, Use of the comet assay to investigate possible interactions of nitric oxide and reactive oxygen species in the induction of DNA damage and inhibition of function in an insulin-secreting cell line Mutation Research. ,vol. 375, pp. 137- 146 ,(1997) , 10.1016/S0027-5107(97)00009-2
Narendra P Singh, Michael T McCoy, Raymond R Tice, Edward L Schneider, None, A simple technique for quantitation of low levels of DNA damage in individual cells Experimental Cell Research. ,vol. 175, pp. 184- 191 ,(1988) , 10.1016/0014-4827(88)90265-0
Tamotsu Inoue, Zhouseng Mu, Kazuhide Sumikawa, Kozaburo Adachi, Toshikazu Okochi, Effect of Physical Exercise on the Content of 8‐Hydroxydeoxyguanosine in Nuclear DNA Prepared from Human Lymphocytes Japanese Journal of Cancer Research. ,vol. 84, pp. 720- 725 ,(1993) , 10.1111/J.1349-7006.1993.TB02035.X
N Zamzami, P Marchetti, M Castedo, D Decaudin, A Macho, T Hirsch, S A Susin, P X Petit, B Mignotte, G Kroemer, Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. Journal of Experimental Medicine. ,vol. 182, pp. 367- 377 ,(1995) , 10.1084/JEM.182.2.367
S. Loft, H. E. Poulsen, Cancer risk and oxidative DNA damage in man Journal of Molecular Medicine. ,vol. 74, pp. 297- 312 ,(1996) , 10.1007/BF00207507
Zsolt Radák, Takao Kaneko, Shoichi Tahara, Hideko Nakamoto, Hideki Ohno, Mária Sasvári, Csaba Nyakas, Sataro Goto, The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes Free Radical Biology and Medicine. ,vol. 27, pp. 69- 74 ,(1999) , 10.1016/S0891-5849(99)00038-6
Marlies De Boeck, Nadia Touil, Geofrey De Visscher, Peter Aka Vande, Micheline Kirsch-Volders, Validation and implementation of an internal standard in comet assay analysis Mutation Research/Genetic Toxicology and Environmental Mutagenesis. ,vol. 469, pp. 181- 197 ,(2000) , 10.1016/S1383-5718(00)00075-9
Andreas Hartmann, Ulla Plappert, Klaus Raddata, Martina Grünert-Fuchs, Günter Speit, Does physical activity induce DNA damage Mutagenesis. ,vol. 9, pp. 269- 272 ,(1994) , 10.1093/MUTAGE/9.3.269
D L Costill, W J Fink, Plasma volume changes following exercise and thermal dehydration. Journal of Applied Physiology. ,vol. 37, pp. 521- 525 ,(1974) , 10.1152/JAPPL.1974.37.4.521