Western Blot Analysis
作者： Seishiro Hirano
DOI: 10.1007/978-1-62703-002-1_6
关键词: Polyacrylamide gel electrophoresis 、 Biology 、 Molecular biology 、 Blot 、 Western blot 、 Primary and secondary antibodies 、 Antibody 、 Dot blot 、 Electrophoresis 、 Northwestern blot
摘要: Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells tissues exposed nanoparticles or nanomaterials. Proteins should be extracted from using a proper method, especially when phosphorylated proteins detected. It is important select good blocking agent an appropriate pair of primary peroxidase-tagged secondary antibodies obtain results analysis. One thing that may specific nanomaterials, you keep mind, some adsorbed on surface particulate In this chapter whole process analysis, sample preparation quantitative measurement target proteins, described.
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