Epidermal and hair follicle transglutaminases. Partial characterization of soluble enzymes in newborn mouse skin.

摘要: Treatment of skins newborn mice with the neutral protease Dispase in order to separate dermis and epidermis causes pronounced changes levels transglutaminase activity epidermis. Two soluble transglutaminases, one anionic enzyme cationic enzyme, Mr approximately 90,000 50,000, respectively, are extracted from epidermis; activities both enzymes increase as a function time treatment skin. When is incubated after its chromatographic isolation epidermal extracts, it converted lower molecular weight enzyme. Hair follicles isolated prepared by 12-h skin contain two which indistinguishable that other not seen Both these hair follicle 50,000 appear exist monomeric form. They have been partially purified. Based upon findings, we suggest processing control occur during normal differentiation keratinocytes cells production proper forms may be essential formation mature cornified envelopes shafts, respectively.