Molecular subtyping and erythromycin resistance of Campylobacter in China

作者: A. Zhang , L. Song , H. Liang , Y. Gu , C. Zhang

DOI: 10.1111/JAM.13135

关键词: Multilocus sequence typingGeneErythromycinCampylobacter coliCampylobacterCampylobacter jejuniGenetics23S ribosomal RNAMicrobiologyBiologyPolymerase chain reaction

摘要: Aims To investigate the erythromycin resistance patterns and mechanism for Campylobacter isolates in China. Methods Results The minimum inhibitory concentrations of on 858 Chinese were analysed. PCR DNA sequencing used to identify mutations 23S rRNA presence ermB gene 158 (18·4%). About 83% (131/158) had A2075G mutation their rRNA; no A2074C/G mutants found. The was identified 30 coli (19%). Four types multidrug-resistant islands (MDRGIs) Fifty-three by multilocus sequence typing among resistant isolates. All STs 6322 1145 gene. Conclusions The rate Camp. coli (58·56%) much higher than jejuni (0·67%). insertion sites between cadF CCO1582 nfsB cinA chromosome might be hot spots MDRGI transformation. Significance Impact Study Point domain V accounted 100% China.

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