A gas-chromatographic headspace method for the determination of acetone in bovine milk, blood and urine.

摘要: An automated headspace gas-chromatographic method has been developed for the determination of acetone in milk, blood and urine dairy cows. Five ml samples were saturated with 2 g sodium chloride equilibrated 30 min at 90 degrees C a Hewlett-Packard HP 19395 A automatic sampler. The volatiles transferred without splitting to 25 m x 0.3 mm 0.4 microns Carbowax column Shimadzu GC 9A gas chromatograph, operating isothermally 50 C. coefficients variation 1.5-4.4% urine, 10.0-24.9% milk 2.0-19.6% blood. detection limits 0.0055 mg/100 0.0072 0.0080 urine. analysis time 5 per sample provided an adequate rate throughput routine monitoring.