Method and kit for detecting chromosome rearrangement
作者： Ding Chunming , Shang Qingjuan , Jin Shengnan
DOI:
关键词: Primer (molecular biology) 、 Molecular biology 、 DNA 、 Chemistry 、 Normal control 、 Chromosomal rearrangement 、 Real-time polymerase chain reaction
摘要: The invention relates to a method and kit for detecting chromosome rearrangement, specifically rearrangement. comprises the following steps: obtainingsample DNA from sample subject; carrying out long-distance PCR (LD-PCR) quantitative (qPCR) on of sample; comparing detection result with normal control sample, deducing whether chromosomal rearrangement is present in according comparison, wherein LD-PCR qPCR can be carried successively orin same reaction container. also kit, primer probe method.
lens.org 参考文章(13)
Julian Lucas, Guochun Liao, Wei Zhou, Filip Crnogorac, Rui Mei, Methods and systems for sequencing long nucleic acids ,(2012)
Tzu-Yu Pan, Chun-Chi Wang, Chi-Jen Shih, Hui-Fen Wu, Shyh-Shin Chiou, Shou-Mei Wu, Genotyping of intron 22 inversion of factor VIII gene for diagnosis of hemophilia A by inverse-shifting polymerase chain reaction and capillary electrophoresis. Analytical and Bioanalytical Chemistry. ,vol. 406, pp. 5447- 5454 ,(2014) , 10.1007/S00216-014-7969-3
Stephen Kingsmore, Sarah Soden, Carol Saunders, Emily G. Farrow, Neil Miller, Laurel K. Willig, System for genome analysis and genetic disease diagnosis ,(2013)
Bai Nan, Hu Shuang, Kong Xiangdong, Mei Shiyue, PCR (polymerase chain reaction) primer for amplifying PKD (polystic kidney disease)1 exon ultra-long fragment, kit for detecting PKD1 gene mutation and application ,(2017)
Wang Xiaowei, Ren Wei, Liu Jingjing, Zhu Yuhua, Severe hemophilia A disease-causing gene mutation detection kit ,(2013)
Li Yinshu, Liu Jingjing, Zhu Yuhua, Heavy hemophilia A FVIII gene intron 22 recombination gene type detection kit ,(2016)
Song Kun, Zhou Xiaodu, Sun Cuilian, Wang Shuyi, Primers, method and kit for detecting inversion of first intron of hemophilia A clotting factor VIII gene ,(2014)
Ding Peifang, Wang Qinyou, Sun Wensheng, Method of biocking bleeder disease A pathogenic gene transfer using fetus umbilical blood ,(2004)