Effects of protein type and composition on postprandial markers of skeletal muscle anabolism, adipose tissue lipolysis, and hypothalamic gene expression

摘要: We examined the acute effects of different dietary protein sources (0.19 g, dissolved in 1 ml water) on skeletal muscle, adipose tissue and hypothalamic satiety-related markers fasted, male Wistar rats (~250 g). Oral gavage treatments included: a) whey concentrate (WPC, n = 15); b) 70:30 hydrolyzed whey-to-hydrolyzed egg albumin (70 W/30E, c) 50 W/50E (n = 15); d) 30 W/70E e) water with no as a fasting control (CTL, n = 14). Skeletal muscle analyses revealed that compared to CTL: phosphorylated (p) mTOR signaling [p-mTOR (Ser2481) p-rps6 (Ser235/236)] were elevated 2–4-fold all groups 90 min post-treatment (p < 0.05); WPC 70 W/30E increased synthesis (MPS) 104% 74% 180 min post-treatment, respectively p-AMPKα (Thr172) 90 180-min well PGC-1α mRNA post-treatment. Subcutaneous (SQ) omental fat (OMAT) revealed: 70 W/30 W SQ hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold versus CTL 1.9–4.4-fold change other test proteins WPC, OMAT p-HSL 3.8–6.5-fold (p < 0.05). POMC suggesting response may have occurred former groups. However, there was compensatory increase orexigenic AGRP group rats, NPY rats. Higher amounts stimulate greatest anabolic response, though higher WPH more favorably affected related lipolysis.