Fabrication of hydrogenase–cationic electrolyte biohybrids at interfaces and their electrochemical properties in Langmuir–Blodgett films

摘要: Abstract Hydrogenase (H2ase)–cationic electrolyte biohybrids were assembled at the air–water interface via intermolecular electrostatic interaction. The H2ase used was purified from phototropic bacterium of Thiocapsa roseopersicina. Two kinds cationic compounds (CECs) used, difference which whether they contained viologen substituent or not. Surface pressure–area isotherms indicated that these CECs co-existed with in monolayers, then transferred to substrate surfaces form H2ase–CECs hybrid films by Langmuir–Blodgett (LB) method. Uniform film formed when polyelectrolyte as subphase. Cyclic voltammograms (CVs) LB showed a couple redox waves potential range −0.4 −0.65 V vs. Ag/AgCl, ascribed one electron process either [4Fe–4S] clusters viologens CECs. A direct transfer between and electrode surface achieved films. Stronger current intensity recorded CV measurements done H2 saturated solution than Ar. It confirmed biocatalytic activity remained Thus, we suggest present could act materials for studies interconversion reaction protons.