αvβ5/β6 integrin suppression leads to a stimulation of α2β1 dependent cell migration resistant to PI3K/Akt inhibition

摘要: Crosstalk between integrins is involved in the regulation of various cell functions including migration. Here we identify interplay alpha v beta 5/beta 6 and 2 1 during migration toward type I collagen. Human colon cancer lines HT29-D4 SW480 were used as models. To improve our Understanding consequences function on 1, decreased expression by either siRNA or lysosomal targeting strategies, inhibited their using, antagonists, blocking antibodies disintegrins. In all cases, observed a greatly enhanced integrin-dependent associated with focal adhesion rearrangements increased outside-in signaling demonstrated elevated phosphorylation kinase MAPKinase (ERK1 ERK2). The 6-dependent limitation could be overridden TS2/16, an activating anti-beta antibody. Interestingly, compared to control cells, pharmacological inhibition PI3Kinase siRNA-mediated knockdown AKT had little effect high 1-mediated absence following activation TS2/16. These results suggest that repress possibly interfering process thereby modify (C) 2009 Elsevier Inc. All rights reserved.