Results of the First World Health Organization International Collaborative Study of Detection of Human Papillomavirus DNA

摘要: Twenty-nine laboratories in 12 countries participated a study to assess the performance of various human papillomavirus (HPV) detection assays through use recombinant HPV DNA standard reagent panel. The panel was designed by group experts, and samples were prepared distributed World Health Organization International Laboratory for Standards Biologicals Netherlands. Each consisted 24 coded including dilution series types 16 18, alone or combination with five other high-risk (HR) 31, 33, 35, 45, 52, low-risk type 6, negative control. Qualitative generally consistent across laboratories, most invalid results reflected lack test sensitivity. combined data sets had proficiency 62.5% (15/24) 18 73.9% (17/23). 31 least accurately detected participating laboratories. Approximately half failed detect high concentrations and, lesser extent, 6. sample materials offer source renewable reproducible material that could be used future development international reagents calibration kits.