Preparation of Pichia pastoris expression plasmids.
作者： Christel Logez , Fatima Alkhalfioui , Bernadette Byrne , Renaud Wagner
DOI: 10.1007/978-1-61779-770-5_3
关键词: Computer science 、 Expression vector 、 Transformation (genetics) 、 Plasmid 、 Promoter 、 Selection (genetic algorithm) 、 Computational biology 、 Heterologous expression 、 Pichia pastoris 、 Membrane protein
摘要: When planning any heterologous expression experiment, the very first critical step is related to design of overall strategy, hence selection most adapted vector. The flexible Pichia pastoris system offers a broad range possibilities for production secreted, endogenous or membrane proteins thanks combination various plasmid backbones, markers, promoters and fusion sequences introduced into dedicated host strains. present chapter provides some guidelines on choice vectors strategies. It also brings reader complete toolbox from which plasmids can be picked assembled set up appropriate vectors. Finally, it standard starting protocols preparation selected their use strain transformation.
springer.com
imperial.ac.uk
springerlink.com参考文章(48)
Gerhard Fischer, Urszula Kosinska-Eriksson, Camilo Aponte-Santamaría, Madelene Palmgren, Cecilia Geijer, Kristina Hedfalk, Stefan Hohmann, Bert L. de Groot, Richard Neutze, Karin Lindkvist-Petersson, Crystal Structure of a Yeast Aquaporin at 1.15 Å Reveals a Novel Gating Mechanism PLoS Biology. ,vol. 7, pp. e1000130- 13 ,(2009) , 10.1371/JOURNAL.PBIO.1000130
Oriol Cos, Ramón Ramón, José Montesinos, Francisco Valero, Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review Microbial Cell Factories. ,vol. 5, pp. 17- 17 ,(2006) , 10.1186/1475-2859-5-17
Romaan J. M. Raemaekers, Laura de Muro, John A. Gatehouse, Anthony P. Fordham-Skelton, Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. FEBS Journal. ,vol. 265, pp. 394- 403 ,(1999) , 10.1046/J.1432-1327.1999.00749.X
Franz S Hartner, Anton Glieder, Regulation of methanol utilisation pathway genes in yeasts Microbial Cell Factories. ,vol. 5, pp. 39- 39 ,(2006) , 10.1186/1475-2859-5-39
S B Ellis, P F Brust, P J Koutz, A F Waters, M M Harpold, T R Gingeras, Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Molecular and Cellular Biology. ,vol. 5, pp. 1111- 1121 ,(1985) , 10.1128/MCB.5.5.1111
J M Cregg, K J Barringer, A Y Hessler, K R Madden, Pichia pastoris as a host system for transformations. Molecular and Cellular Biology. ,vol. 5, pp. 3376- 3385 ,(1985) , 10.1128/MCB.5.12.3376
Franz S. Hartner, Claudia Ruth, David Langenegger, Sabrina N. Johnson, Petr Hyka, Geoffrey P. Lin-Cereghino, Joan Lin-Cereghino, Karin Kovar, James M. Cregg, Anton Glieder, Promoter library designed for fine-tuned gene expression in Pichia pastoris Nucleic Acids Research. ,vol. 36, ,(2008) , 10.1093/NAR/GKN369
Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: pharmacological characterization and localization. FEBS Letters. ,vol. 377, pp. 451- 456 ,(1995) , 10.1016/0014-5793(95)01389-X
Tomoshi Ohya, Toyoo Ohda, Masao Ohyama, Kenji Tomomitsu, Kaoru Kobayashi, Shinobu Kuwae, Addition of oleic acid increases expression of recombinant human serum albumin by the AOX2 promoter in Pichia pastoris Journal of Bioscience and Bioengineering. ,vol. 89, pp. 479- 484 ,(2000) , 10.1016/S1389-1723(00)89100-8