In vitro interactions of c-ANCA (antibodies to proteinase 3) with human endothelial cells.
作者： Werner-J. Mayet , Elisabeth M. Hermann , Elena Csernok , Wolfgang L. Gross , Karl-H. Meyer zum Büschenfelde
DOI: 10.1007/978-1-4757-9182-2_18
关键词: Molecular biology 、 In vitro 、 Monoclonal antibody 、 Antibody 、 Biology 、 Anti-neutrophil cytoplasmic antibody 、 Western blot 、 Proteinase 3 、 Cytoplasm 、 C-ANCA
摘要: Several concepts concerning the pathogenicity of antineutrophil cytoplasmic antibodies (ANCA) exist, but till now only sparse data about ANCA-endothelial interactions are available. In this study we have investigated expression proteinase 3 (PR-3) in human umbilical endothelial cells (HEC) using purified anti-PR3 (C-ANCA) patients with Wegener’s granulomatosis (WG) and monoclonal to PR-3 (human murine) as probes. Performing cytoELISAs, laser scanning microscopy Western blot were able show that treatment HEC IL-1-alpha led an increased cytoplasm a transient translocation into EC-membrane. Representing important missing link interactions, our give hint at possible direct anti-PR-3 WG other vasculitides.
springer.com
sci-hub.st 参考文章(8)
L A van Es, F J van der Woude, M R Daha, The current status of neutrophil cytoplasmic antibodies. Clinical and Experimental Immunology. ,vol. 78, pp. 143- 148 ,(1989)
E. Csernok, D. F. Bainton, W. L. Gross, J. Lüdemann, Ultrastructural localization of proteinase 3, the target antigen of anti-cytoplasmic antibodies circulating in Wegener's granulomatosis. American Journal of Pathology. ,vol. 137, pp. 1113- 1120 ,(1990)
S. Thornton, S. Mueller, E. Levine, Human endothelial cells: use of heparin in cloning and long-term serial cultivation Science. ,vol. 222, pp. 623- 625 ,(1983) , 10.1126/SCIENCE.6635659
Werner J. Mayet, Elisabeth Hermann, Bernd Kiefer, Harald Lehmann, Michael Manns, Karl H. Meyer Zum Büschenfelde, In vitro production of anti-neutrophilocyte-cytoplasm-antibodies (ANCA) by Epstein-Barr virus-transformed B-cell lines in Wegener's granulomatosis. Autoimmunity. ,vol. 11, pp. 13- 19 ,(1991) , 10.3109/08916939108994703
Werner-J. Mayet, Georg Hess, Guido Gerken, Siegbert Rossol, Rita Voth, Michael Manns, Karl-Hermann Meyer ZumBüschenfelde, Treatment of chronic type B hepatitis with recombinant α-interferon induces autoantibodies not specific for autoimmune chronic hepatitis Hepatology. ,vol. 10, pp. 24- 28 ,(1989) , 10.1002/HEP.1840100106
G. FRAMPTON, D. R. W. JAYNE, G. J. PERRY, C. M. LOCKWOOD, J. S. CAMERON, Autoantibodies to endothelial cells and neutrophil cytoplasmic antigens in systemic vasculitis. Clinical and Experimental Immunology. ,vol. 82, pp. 227- 232 ,(2008) , 10.1111/J.1365-2249.1990.TB05431.X
Eric A. Jaffe, Ralph L. Nachman, Carl G. Becker, C. Richard Minick, Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIA Journal of Clinical Investigation. ,vol. 52, pp. 2745- 2756 ,(1973) , 10.1172/JCI107470
Justine S. Garvey, Methods in immunology ,(1977)