Expression and Purification of Recombinant Proteins Using the pET System.

作者: Robert C. Mierendorf , Barbara B. Morris , Beth Hammer , Robert E. Novy

DOI: 10.1385/0-89603-485-2:257

关键词: Cell biologyBiologyTranscription (biology)GenePlasmidCloningEscherichia coliBacteriophageLacUV5T7 RNA polymerase

摘要: … pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET … of this system is its …

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