Specific structural alteration of the influenza haemagglutinin by amantadine.

摘要: Amantadine hydrochloride specifically blocks the release of virus particles from H7 influenza infected cells. This appears to be direct consequence an amantadine induced change in haemagglutinin (HA) its low pH conformation. The effect is indirect and mediated via interaction drug with M2 protein since mutants altered this component alone are insensitive amantadine. timing action, some 15-20 min after synthesis, coincidence proteolytic cleavage indicates that modifications HA occur late during transport but prior insertion into plasma membrane. Reversal by mM concentrations amines 0.1 microM monensin action causes a reduction intravesicular which triggers conformational HA. We conclude, therefore, function inhibited involved counteracting acidity vesicular compartments exocytic pathway cells important protecting structural integrity acid-sensitive glycoprotein.