MAGPIE/EGRET annotation of the 2.9-Mb Drosophila melanogaster Adh region.
作者： Terry Gaasterland , Alexander Sczyrba , Elizabeth Thomas , Gulriz Aytekin-Kurban , Paul Gordon
DOI: 10.1101/GR.10.4.502
关键词: Genome 、 Genetics 、 Computational biology 、 Biology 、 Whole genome sequencing 、 Exon 、 Drosophila melanogaster 、 Complementary DNA 、 Genome evolution 、 Annotation 、 DNA
摘要: Our challenge in annotating the 2.91-Mb Adh region of Drosophila melanogaster genome was to identify genetic and genomic features automatically, completely, precisely within a 6-week period. To do so, we augmented MAGPIE microbial annotation system handle eukaryotic sequence data. The new configuration required integration gene-finding tools DNA repeat into automatic data collection module. It also us define strategies combine about exon predictions with functional refine predictions. At heart resulting is reverse comparison public protein complementary sequences against input missing exons boundaries. software modules that add capability are available as EGRET (Eukaryotic Genome Rapid Evaluation Tool).
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