Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification.
作者： Christian Bellehumeur , Brian Boyle , Steve J. Charette , Josée Harel , Yvan L’Homme
DOI: 10.1016/J.JVIROMET.2015.06.014
关键词: Respiratory virus 、 Porcine reproductive and respiratory syndrome virus 、 Nucleic acid 、 Biology 、 Propidium monoazide 、 Enzyme 、 RNA 、 Ethidium bromide 、 Reverse transcription polymerase chain reaction 、 Molecular biology 、 Microbiology
摘要: Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel viruses of emerging diseases. However, both techniques have difficulties to identify …
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