An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits.
作者： Shang Xie , Hui Xu , Bo Lin , Kan Wang , Xiao-Feng Shan
DOI: 10.1155/2016/7058537
关键词: Submandibular gland 、 Western blot 、 Transplantation 、 Staining 、 Internal medicine 、 Secretion 、 Medicine 、 Endocrinology 、 Immunofluorescence 、 H&E stain 、 Cytoplasm
摘要: Objectives. To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms. Methods. new SMG model was established rabbit. 30 successfully constructed models were randomly assigned to five groups including group four experimental groups. Secretion outputs used analyze the effect of BTXA injection on secretion. Hematoxylin eosin (HE) staining, transmission electron microscopy (TEM), Western blot, immunofluorescence performed Results. After injection, a significant decrease found 2 4 weeks groups, but no 12 24 HE TEM results showed that led morphological ultrastructural changes acinar cells transplanted SMG. blot suggested decreased aquaporin-5 (AQP5) protein expression for weeks. Immunofluorescence AQP5 mainly expressed cytoplasm weeks, which might indicate promoted from cell membrane cytoplasm. Conclusion. effectively rabbits.
hindawi.com
core.ac.uk 参考文章(41)
Shang Xie, Kan Wang, Tao Xu, Xue‐Sheng Guo, Xiao‐Feng Shan, Zhi‐Gang Cai, Efficacy and safety of botulinum toxin type A for treatment of Frey's syndrome: evidence from 22 published articles Cancer Medicine. ,vol. 4, pp. 1639- 1650 ,(2015) , 10.1002/CAM4.504
Xi-yuan Ge, Guang-yan Yu, Jia Fu, Deng-cheng Wu, Xiao-xia Zhang, Yi-xiang Wang, Sheng-lin Li, An experimental study of the management of severe keratoconjunctivitis sicca with autologous reduced-sized submandibular gland transplantation British Journal of Oral and Maxillofacial Surgery. ,vol. 50, pp. 562- 566 ,(2012) , 10.1016/J.BJOMS.2011.10.004
Gerd Geerling, John R. Garrett, Katherine L. Paterson, Peter Sieg, J Richard O. Collin, Guy H. Carpenter, Samer G. Hakim, Isabel Lauer, Gordon B. Proctor, Innervation and Secretory Function of Transplanted Human Submandibular Salivary Glands Transplantation. ,vol. 85, pp. 135- 140 ,(2008) , 10.1097/01.TP.0000296060.39823.A4
Ankita S Bhavsar, Samir G Bhavsar, Sunita M Jain, None, A review on recent advances in dry eye: Pathogenesis and management Oman Journal of Ophthalmology. ,vol. 4, pp. 50- 56 ,(2011) , 10.4103/0974-620X.83653
Yasuko Ishikawa, Hajime Ishida, Aquaporin water channel in salivary glands. Japanese Journal of Pharmacology. ,vol. 83, pp. 95- 101 ,(2000) , 10.1254/JJP.83.95
P.A. Vinod Kumar, Michael J. Hickey, Chandan J. Gurusinghe, Bernard McC. O'Brien, Long term results of submandibular gland transfer for the management of xerophthalmia British Journal of Plastic Surgery. ,vol. 44, pp. 506- 508 ,(1991) , 10.1016/0007-1226(91)90006-6
Norio Ohba, The 101 most frequently cited articles in ophthalmology journals from 1850 to 1949. Archives of Ophthalmology. ,vol. 128, pp. 1610- 1617 ,(2010) , 10.1001/ARCHOPHTHALMOL.2010.308
Jian-Rui Cai, Xiao-Feng Shan, Zhi-Gang Cai, Xuan Zhang, Guang-Yan Yu, A New Treatment for Epiphora Secondary to Submandibular Gland Transplantation: Transcutaneous Atropine Gel Ocular Surface. ,vol. 12, pp. 221- 226 ,(2014) , 10.1016/J.JTOS.2014.04.001
Hans-Christian Jacobsen, Samer G. Hakim, Isabel Lauer, Andreas Dendorfer, Thilo Wedel, Peter Sieg, Long-term results of autologous submandibular gland transfer for the surgical treatment of severe keratoconjunctivitis sicca. Journal of Cranio-maxillofacial Surgery. ,vol. 36, pp. 227- 233 ,(2008) , 10.1016/J.JCMS.2007.08.010
L Shi, X Cong, Y Zhang, C Ding, QW Ding, FY Fu, LL Wu, GY Yu, Carbachol improves secretion in the early phase after rabbit submandibular gland transplantation. Oral Diseases. ,vol. 16, pp. 351- 359 ,(2010) , 10.1111/J.1601-0825.2009.01633.X