Novel polymer-polyphenol beads for encapsulation and microreactor applications

摘要: Novel structures with controllable morphology made from natural materials hold promise for several applications in pharmaceuticals, foods and biotechnology field. In this paper, we utilized the strong interactions between epigallocatechin gallate (EGCG), a polyphenol obtained green tea, water soluble cellulose ethers like methyl (MCE) hydroxyl propyl methylcellulose (HPMC) to fabricate novel beads core–shell morphology. The polymer–polyphenol were prepared by simply adding polymer solution dropwise into an aqueous of polyphenol. Effect process variables concentration viscosity grades polymer, reaction time EGCG on bead formation was evaluated. Spherical milky white average diameters ranging 600–2500 µm that retained shape after freeze drying. Internal structure studied using automated light microscopy cold stage scanning electron (Cryo-SEM). Percent loading above 25% w/w all cases. release study simulated intestinal fluid revealed burst (20% first 5 minutes) followed sustained more than 90% at end 300 minutes. antioxidant power effectively maintained alkaline conditions 5–6 fold activity as compared free EGCG. Further, temperature triggered characteristic explored encapsulating eucalyptus oil studying hot microscopy. Enzyme immobilisation application demonstrated immobilising α-amylase onto its enzyme washings.