Dominant negative mutant of c-Jun inhibits NF-AT transcriptional activity and prevents IL-2 gene transcription.

摘要: Expression of the transcription complex AP-1, composed Jun and Fos family members, can be induced by a variety stimuli. In lymphocytes, AP-1 transcriptional activity increases after TCR ligation plays an important role in T cell activation events such as lymphokine secretion. To explore requirements for IL-2 production, was targeted with dominant negative mutant c-Jun protein, TAM-67, from which transactivation domain has been deleted. transient transfections Jurkat cells, TAM-67 efficiently inhibited endogenous blocked reporter construct containing 5' regulatory region gene. also nuclear factor-AT (NF-AT), whereas NF-kappa B, NF-IL-2A, proximal TRE-like sites were relatively unaffected. The use this factor suggests that: 1) transactivation-defective factors represent novel approach to study functional consequences protein interactions on gene transcription; 2) site promoter is different consensus TRE; 3) mediated NF-AT binding complex.