Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control
作者： Alexander Zilbershtein , Amir Bein , Vladislav Lirtsman , Betty Schwartz , Michael Golosovsky
DOI: 10.1117/1.JBO.19.11.111608
关键词: Monolayer 、 Biophysics 、 Nuclear magnetic resonance 、 Surface plasmon resonance 、 Fourier transform spectroscopy 、 Infrared spectroscopy 、 Localized surface plasmon 、 Materials science 、 Polarization (electrochemistry) 、 Biosensor 、 Surface plasmon
摘要: We report a label-free infrared surface plasmon biosensor with double-chamber flow cell for continuous monitoring of morphological changes in culture exposed to various stimuli. In this technique, the monolayer cultured cells is divided into two halves by barrier, allowing treatment one half while other serves as control. demonstrate advantages setup test experiments that track kinetics IEC-18 layer response variations extracellular Ca2+ concentration. The sensitivity presented method was found be an order magnitude higher compared single-chamber biosensor.
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