The Saccharomyces cerevisiae chromosome III left telomere has a type X, but not a type Y', ARS region.

摘要: Abstract A yeast Saccharomyces cerevisiae telomeric region was isolated by chromosome walking from HML alpha, the most distal known gene on III left (IIIL) end. The terminal heterodisperse 3.3-kilobase (kb) SalI fragment IIIL, 8.6 kb to cloned in a circular vector generate probe. Southern hybridization and DNA sequencing analyses indicated that 0.6 (+/- 200 base pairs) of 5'-C1-3A-3' simple tandem repeat sequence, adjacent 1.2-kb type X ARS region, constitutes telomere IIIL end, no Y' homologies exist between alpha terminus.