Regulation of peri-attachment embryo development in the golden hamster: role of growth factors

作者: P.B Seshagiri , A Mishra , G Ramesh , R.P Rao

DOI: 10.1016/S0165-0378(01)00086-9

关键词: EndocrinologyInternal medicineEpidermal growth factorParacrine signallingTransforming growth factorLeukemia inhibitory factorGrowth factorTrophoblastBiologyBlastocystHamsterCell biology

摘要: The molecular regulation of mammalian peri-implantation development is complex and difficult to study in vivo. We successfully cultured hamster blastocysts through hatching peri-attachment stages, using a chemically defined medium, HECM-2h. Using this system, we showed that species-specific, embryonic cysteine-like protease involved blastocyst the process modulated by growth factors. In particular, heparin binding-epidermal factor (HB-EGF) or leukemia inhibitory (LIF) enhance hatching, former also improves attachment trophoblast outgrowth. observed interesting changing patterns expression mRNA and/or immunoreactive protein for EGF, HB-EGF, LIF transforming factor-β (TGF-β) embryo endometrial tissue, during development. Together, it appears outgrowth are regulated autocrine paracrine factors, produced embryo-endometrial tissues.

参考文章(41)
Hans-Werner Denker, Implantation. The role of proteinases, and blockage of implantation by proteinase inhibitors. Advances in Anatomy Embryology and Cell Biology. ,vol. 53, pp. 3- 123 ,(1977)
H. Tamada, C. Higashiyama, H. Takano, N. Kawate, T. Inaba, T. Sawada, The effects of heparin-binding epidermal growth factor-like growth factor on preimplantation-embryo development and implantation in the rat. Life Sciences. ,vol. 64, pp. 1967- 1973 ,(1999) , 10.1016/S0024-3205(99)00128-9
Barry D. Bavister, Culture of preimplantation embryos: facts and artifacts Human Reproduction Update. ,vol. 1, pp. 91- 148 ,(1995) , 10.1093/HUMUPD/1.2.91
David S. Gonzales, Dorothy E. Boatman, Barry D. Bavister, Kinematics of trophectoderm projections and locomotion in the peri-implantation hamster blastocyst. Developmental Dynamics. ,vol. 205, pp. 435- 444 ,(1996) , 10.1002/(SICI)1097-0177(199604)205:4<435::AID-AJA7>3.0.CO;2-J
Alfred R. Menino, Jane S. Williams, Activation of plasminogen by the early bovine embryo. Biology of Reproduction. ,vol. 36, pp. 1289- 1295 ,(1987) , 10.1095/BIOLREPROD36.5.1289