Secrets of Successful Embedding, Sectioning, and Imaging

摘要: For those of us who have worked with resistant structures such as spores, embedding media always been central to success in ultrathin sectioning. Development an effective protocol often meant or failure a research project. However, microbiologists gained more experience variety resins, it has become clear that we must address criteria beyond penetration. Choice resin can affect image contrast, stability under the electron beam, granularity, and size cells organelles. Furthermore, when postsectioning manipulations immunocytochemistry colloidal gold are used, consider characteristics hydrophobicity charge on sections select protocols.