Co-outbreak of ST37 and a novel ST3006 Klebsiella pneumoniae from multi-site infection in a neonatal intensive care unit: a retrospective study
作者： Dongjie Chen , Xinlan Hu , Falin Chen , Hongru Li , Daxuan Wang
DOI: 10.1101/334169
关键词: Pulsed-field gel electrophoresis 、 Multilocus sequence typing 、 Intensive care 、 Antibiotic resistance 、 Biology 、 Drug resistance 、 Virulence 、 Neonatal intensive care unit 、 Klebsiella pneumoniae 、 Microbiology
摘要: Background: The spread of carbapenem resistance among Klebsiella pneumoniae (K. pneumoniae) is a major public health problem, particularly in neonatal intensive care units (NICUs). Aim: To describe the nosocomial co-outbreak ST37 and new sequence type ST3006 K. pneumoniae, causing catheter-related bloodstream infections NICU. Methods: Fifteen strains were isolated from seven neonates during June 3-28, 2017, tertiary hospital unit Fujian, China. Antimicrobial susceptibility was determined by Vitek 2 system micro-broth dilution method. Multi-locus typing (MLST) pulsed-field gel electrophoresis (PFGE) used to analyse genetic relatedness isolates. Genome sequencing gene function analyses performed for investigating pathogenicity drug screening genomic islands. Findings: Two clones identified with multi-site infection, one strain ST3006. testing revealed that exhibited multi-drug resistance. only resistant ampicillin-sulbactam, cefazolin, ceftriaxone. MLST PFGE showed 15 divided into three groups, high level homology. Gene analysis indicated KPN1343 harboured 12 genes, islands 205 reduced virulence genes. KPN1344 four 19 209 Conclusion: Co-outbreak involved two clones, ST36 ST3006, infection. an effective method studying bacterial genes their functions.
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