FAM46B inhibits cell proliferation and cell cycle progression in prostate cancer through ubiquitination of β-catenin
作者： Tao Liang , Xuxiao Ye , Yuanyuan Liu , Xinkai Qiu , Zuowei Li
DOI: 10.1038/S12276-018-0184-0
关键词: Gene silencing 、 Carcinogenesis 、 Cell growth 、 Cyclin D1 、 Cell culture 、 Chemistry 、 Flow cytometry 、 Catenin 、 Cancer research 、 Cell cycle
摘要: FAM46B is a member of the family with sequence similarity 46. Little known about expression and functional role(s) in prostate cancer (PC). In this study, The Cancer Genome Atlas, GSE55945, an independent hospital database was measured by bioinformatics real-time PCR analysis. After PC cells were transfected siRNA or recombinant vector absence presence β-catenin signaling inhibitor (XAV-939), levels FAM46B, C-myc, Cyclin D1, western blot PCR. Cell cycle progression cell proliferation flow cytometry CCK-8 assay. effects on tumor growth protein nude mice xenografts also measured. Our results showed that downregulated but upregulated patients PC. silencing promoted PC, which abrogated XAV-939. Moreover, overexpression inhibited vitro vivo. through inhibition ubiquitination. data clearly show inhibits ubiquitination β-catenin.
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sci-hub.se 参考文章(39)
Thitima Benjachat, Pumipat Tongyoo, Pornpen Tantivitayakul, Poorichaya Somparn, Nattiya Hirankarn, Santitham Prom-On, Prapaporn Pisitkun, Asada Leelahavanichkul, Yingyos Avihingsanon, Natavudh Townamchai, Biomarkers for Refractory Lupus Nephritis: A Microarray Study of Kidney Tissue. International Journal of Molecular Sciences. ,vol. 16, pp. 14276- 14290 ,(2015) , 10.3390/IJMS160614276
Godfrey Essien Etokebe, Shanbeh Zienolddiny, Zeljko Kupanovac, Morten Enersen, Sanja Balen, Veljko Flego, Ljiljana Bulat-Kardum, Anđelka Radojčić-Badovinac, Vidar Skaug, Per Bakke, Aage Haugen, Zlatko Dembic, Association of the FAM46A Gene VNTRs and BAG6 rs3117582 SNP with Non Small Cell Lung Cancer (NSCLC) in Croatian and Norwegian Populations PLOS ONE. ,vol. 10, pp. e0122651- ,(2015) , 10.1371/JOURNAL.PONE.0122651
T Martino, F C J Magalhães, G. A. JUSTO, M. G. P. COELHO, C.D. Netto, P.R.R. Costa, K. C. C. SABINO, The pterocarpanquinone LQB-118 inhibits tumor cell proliferation by downregulation of c-Myc and cyclins D1 and B1 mRNA and upregulation of p21 cell cycle inhibitor expression. Bioorganic & Medicinal Chemistry. ,vol. 22, pp. 3115- 3122 ,(2014) , 10.1016/J.BMC.2014.04.025
Cosetta Bertoli, Jan M. Skotheim, Robertus A. M. de Bruin, Control of cell cycle transcription during G1 and S phases Nature Reviews Molecular Cell Biology. ,vol. 14, pp. 518- 528 ,(2013) , 10.1038/NRM3629
N. R. Gough, Focus issue: Wnt and β-catenin signaling in development and disease. Science Signaling. ,vol. 5, pp. 2002806- ,(2012) , 10.1126/SCISIGNAL.2002806
Seth M. Rubin, Deciphering the retinoblastoma protein phosphorylation code Trends in Biochemical Sciences. ,vol. 38, pp. 12- 19 ,(2013) , 10.1016/J.TIBS.2012.10.007
Hans Clevers, Roel Nusse, Wnt/β-catenin signaling and disease. Cell. ,vol. 149, pp. 1192- 1205 ,(2012) , 10.1016/J.CELL.2012.05.012
Angel Pascual, Ana Aranda, Thyroid hormone receptors, cell growth and differentiation Biochimica et Biophysica Acta. ,vol. 1830, pp. 3908- 3916 ,(2013) , 10.1016/J.BBAGEN.2012.03.012
Isabel Chu, Jun Sun, Angel Arnaout, Harriette Kahn, Wedad Hanna, Steven Narod, Ping Sun, Cheng-Keat Tan, Ludger Hengst, Joyce Slingerland, p27 Phosphorylation by Src Regulates Inhibition of Cyclin E-Cdk2 Cell. ,vol. 128, pp. 281- 294 ,(2007) , 10.1016/J.CELL.2006.11.049
W. Michael Kuehl, P. Leif Bergsagel, Molecular pathogenesis of multiple myeloma and its premalignant precursor Journal of Clinical Investigation. ,vol. 122, pp. 3456- 3463 ,(2012) , 10.1172/JCI61188