Cytokinetic basis for the impaired activation of lymphocytes from patients with primary intracranial tumors.

摘要: Patients with malignant brain tumors have a variety of immunologic abnormalities, including the impaired responsiveness peripheral blood lymphocytes (PBL) to mitogens and alloantigens. We further investigated this impairment lymphocyte reactivity by employing techniques limiting dilution analysis cytokinetic analysis. PBL preparations from patients approximately six times fewer phytohemagglutinin (PHA)-responsive cells than normal subjects. Similar results were obtained purified T cell preparations. Cytokinetic PHA-induced [3H]thymidine incorporation colchicine blocking mitosis demonstrated that number first generation entering S-phase for each 24-hr period was less individuals. First responding subjects entered DNA synthesis at same time (48 72 hr). over 168 hr whereas individuals second cells, majority did not, thus indicating defect in their ability undergo clonal expansion. Measurement interleukin 2 (IL 2) activity culture fluids PHA-activated revealed significantly lower IL levels patients. The addition various concentrations lectin-free stimulated PHA not restore values. There no difference between 1 1) produced lipopolysaccharide-activated monocytes Overall, these suggest an intrinsic exists tumor renders them unable enter into mitogen-induced blastogenesis.