CRABP-II- and FABP5-independent all-trans retinoic acid resistance in COLO 16 human cutaneous squamous cancer cells
作者： Nan-Nan Chen , Yan Li , Mo-Li Wu , Zhi-Li Liu , Yuan-Shan Fu
DOI: 10.1111/J.1600-0625.2011.01392.X
关键词: CYP26A1 、 Cancer cell 、 DNA methylation 、 Bisulfite sequencing 、 Cell culture 、 Cell 、 Molecular biology 、 Retinoic acid 、 Transfection 、 Biology
摘要: : The effect of all-trans retinoic acid (ATRA) on cutaneous squamous cell carcinomas (c-SCC) has been poorly described. Because the imbalance CRABP-II-mediated anticancer signalling and FABP5-mediated growth-promoting was supposed to be related with ATRA sensitivities cancer cells, COLO16 human c-SCC line selected check underlying mechanism leading resistance by multiple experimental approaches. results revealed that COLO 16 cells were resistant 15 μm treatment. FABP5 as well elements CRABP-II (CYP26A1, CYP26B1, CRABP-I, RARα/β/γ RXRα/β/γ) (PPARβ/δ) expressed, but undetectable in cells. 5-Aza treatment enhanced expression further bisulfite sequencing PCR-DNA no methylation promoter region. Transfection CRABP-II-expressing plasmids or siRNA both successfully manipulated level(s) target gene failed overcome transfectants. In conclusion, imbalanced ATRA-resistant 5-Aza-enhanced unmethylation region suggest certain regulatory gene(s) As neither restoration nor increased versus ratio can additional mechanism(s) may present c-SCCs would value addressing this issue.
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