Smartphone-interfaced 3D printed toxicity biosensor integrating bioluminescent “sentinel cells”
作者： Luca Cevenini , Maria Maddalena Calabretta , Giuseppe Tarantino , Elisa Michelini , Aldo Roda
DOI: 10.1016/J.SNB.2015.11.017
关键词: Biosensor 、 Android (operating system) 、 Genetically engineered 、 Bioluminescence 、 Computer hardware 、 Computer science 、 3d printer 、 Nanotechnology 、 3d printed 、 Interface (computing) 、 Instrumentation (computer programming)
摘要: Abstract In this work, we report the design, fabrication, and preliminary assessment of analytical performance a smartphone-based bioluminescence (BL) whole-cell toxicity biosensor. Genetically engineered human embryonic kidney cells, constitutively expressing powerful green-emitting luciferase mutant, were used as “sentinel cells” integrated into 3D printed ready-to-use cartridges, also containing assay reagents. Customizable, low-cost smartphone adaptors created using desktop printer to provide mini-darkbox an aligned optical interface between camera cell cartridge for BL signals acquisition. The developed standalone compact device, which includes disposable droppers sample reagents addition, allows user perform within 30 min following procedure provided by custom-developed application running on Android (Tox-App). As proof-of-concept analyzed real samples including ubiquitous products in everyday life. results showed good correlation with those obtained laboratory instrumentation commercially available assays, thus supporting potential applications proposed device portable real-life needs.
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