Lysosomal Accumulation and Recycling of Lipopolysaccharide to the Cell Surface of Murine Macrophages, an In Vitro and In Vivo Study

摘要: In this study, we detailed in a time-dependent manner the trafficking, recycling, and structural fate of Brucella abortus LPS murine peritoneal macrophages by immunofluorescence, ELISA, biochemical analyses. The intracellular pathway B. LPS, nonclassical endotoxin, was investigated both vivo after injection cavity mice vitro incubation with macrophages. We also followed trafficking infection strain 19. After binding to cell surface internalization, is routed from early endosomes lysosomes unusual slow kinetics. It accumulates there for at least 24 h. Later, leaves reaches macrophage surface. This recycling observed released S19 following infection. Indeed, 72 h postinfection, bacteria are degraded located inside dispersed periphery. From onward, gradually detected plasma membrane. each case, present found large clusters O-chain facing extracellular medium. Both antigenicity heterogenicity moiety preserved during trafficking. demonstrate that not cleared either or 3 mo, exposing its immunogenic toward