Rapid isolation of PCR-ready DNA from blood, bone marrow and cultured cells, based on paramagnetic beads

摘要: PCR-based methods for the analysis of genomic DNA are becoming increasingly common both in research and routine purposes. A rapid, small-scale isolation method is needed to take full advantage speed automation potential PCR technology. We demonstrate use Dynabeads DIRECT, a kit PCR-ready from whole blood, bone marrow or cultured cells less than 10 min. The based on adsorption magnetic beads prior separation involves no centrifugation steps organic solvents. yield quality comparable traditional large-scale methods. One enough at least ten PCRs.