DOI:
关键词: Circular dichroism 、 Microemulsion 、 Native state 、 Micelle 、 Chemistry 、 Glycerol 、 Solubility 、 Aqueous solution 、 Pharmaceutical formulation 、 Chromatography
摘要: Objective: Proteins and enzymes are marginally stable macromolecules along with certain additives for converting them into suitable drug formulation. The research in pharmaceuticals claims the suitability of wide variety range micelles, reverse unsaturated fatty acids their esters glycols act as a protective fence such therapeutic against denaturants colloidal form. present study, address preformulation compatibility study different bioenzyme- chondroitinase futuristic microemulsion Methods: Initially solubility excipients was determined to obtain aqueous, oil surfactants phase. FTIR spectra individual each additive binary blend form were compared retain characteristic bands native protein. It followed non-denaturing SDS-PAGE ensure conformation enzyme on gel presence additives. Circular dichroism technique used investigate stability primary secondary structure these mixtures. control accelerated studies performed by spectroscopy. Results: Chondroitinase observed as: phosphate buffer > propylene glycol glycerol Tween 80 Oleic acid. retained blends. ensured no signs protein fragments, aggregation or degradation gel. CD revealed major loss activity. Control confirmed concentration purity blends accepted limit. Conclusion: These results demonstrated that employed this safe Keywords: Dichroism, FTIR-ATR, SDS-PAGE, Preformulation, Enzyme-additive compatibility, Therapeutic c hondroitinase.