Leucine-Rich Repeat Kinase 2 Controls Inflammatory Cytokines Production through NF-κB Phosphorylation and Antigen Presentation in Bone Marrow-Derived Dendritic Cells.

摘要: Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson’s disease. Although characteristics LRRK2 have gradually been revealed, its true physiological functions remain unknown. highly expressed in immune cells such as B2 and macrophages, suggesting that it plays important roles system. In present study, we investigate dendritic (DCs). Bone marrow-derived DCs from both C57BL/6 wild-type (WT) knockout (KO) mice were induced by culture with granulocyte/macrophage-colony stimulating factor (GM/CSF) vitro. We observed differentiation DCs, phosphorylation transcriptional factors NF-κB, Erk1/2, p-38 after lipopolysaccharide (LPS) stimulation antigen-presenting ability flow cytometry. also analyzed production inflammatory cytokines ELISA. During observation period, there was no difference DC between WT LRRK2-KO mice. After LPS stimulation, NF-κB significantly increased KO Large amounts produced infection Leishmania. CD4+ T-cells isolated antigen-immunized proliferated to a greater degree upon coculture antigen-stimulated than These results suggest may play signal transduction antigen presentation DCs.