Enzyme-amplified lanthanide chelate luminescence
作者： Eva F. G. Templeton , Ramon A. Evangelista , Alfred Pollak
DOI:
关键词: Photochemistry 、 Homogeneous 、 Luminescence 、 Substrate (chemistry) 、 Chelation 、 Materials science 、 Enzyme 、 Lanthanide
摘要: A method and compounds useful for this are described enzyme-amplified signal detection in analytical assays requiring extremely high sensitivity which uses a substrate capable of being transformed by an enzyme from compound does not form luminescent lanthanide chelate into product forms chelate. The forming highly is enzymatically altered to produce hence particularly time-resolved luminescence analysis as required many different heterogeneous or homogeneous assay formats herein.
lens.org 参考文章(27)
Emilia F. Barsa, Antonio C. Marin Moga, 2-phosphonoxy-4-trifluoromethylbenzoic acid derivatives and pharmaceutical compositions containing same ,(1976)
Erkki Soini, Timo Lovgren, Ilkka Hemmila, Method for quantitative determination of a biospecific affinity reaction ,(1983)
Irwin Wieder, Method and apparatus for improved analytical fluorescent spectroscopy ,(1975)
Axel Johannsson, David H. Ellis, David L. Bates, Anne M. Plumb, Christopher J. Stanley, Enzyme amplification for immunoassays: Detection limit of one hundredth of an attomole Journal of Immunological Methods. ,vol. 87, pp. 7- 11 ,(1986) , 10.1016/0022-1759(86)90337-6
Thomas Raymond Gingeras, Gary Fred Musso, Soumitra Shankar Ghosh, Lanthanide chelate-tagged nucleic acid probes. ,(1986)
Ramon A. Evangelista, Alfred Pollak, 1,10-phenanthroline derivatives and use in fluorescence immunoassays ,(1985)
Donald M. Crothers, Nanibhushan Dattagupta, Labelled nucleic acid probes and adducts for their preparation ,(1984)
Veli-Matti Mukkala, Ilkka Hemmila, Heikki Mikola, Determination method utilizing reagents covalently labelled with essentially non-fluorescent lanthanide chelates in combination with time-resolved fluorescence spectroscopy and the reagents to be used in the method ,(1987)
John L. Toner, Jerald C. Hinshaw, George A. Reynolds, Fluorescent labels for immunoassay ,(1986)
Thomas E. Shenk, Robert J. Schneider, Amplified hybridization assay ,(1986)