Phospholipid transfer protein mediated conversion of high density lipoproteins generates preβ1-HDL

摘要: High density lipoproteins (HDL) subclasses can be differentiated by two-dimensional non-denaturing polyacrylamide gradient gel electrophoresis (2D-PAGGE) and subsequent immunoblotting. The quantitatively minor HDL-subclasses preβ1-LpA-I γ-LpE are initial acceptors of cell-derived cholesterol into the plasma compartment. In this study we analysed effect phospholipid transfer protein (PLTP) on electrophoretic distribution in as well ability plasma, preβ1-LpA-I, to take up [3H]cholesterol from labeled fibroblasts. Preβ1-LpA-I but not disappeared during a 16 hours incubation absence PLTP. During one minute pre-incubated released 75% less radiolabeled fibroblasts than control plasma. Pre-incubation reduced uptake 40%. Totally, efflux capacity decreased 10% compared original sample. amount immunodetectable increased when was incubated presence PLTP while did change. After PLTP-conditioned with [3H]cholesterol-labeled fibroblasts, radioactive taken twice high whereas remained unchanged. As net result, treatment total Together results previous studies our data suggest that conversion α-LpA-I3 α-LpA-I2 generates γ-LpE. helps enhance preβ1-LpA-1 and, thereby, normal