Variation among Genome Sequences of H37Rv Strains of Mycobacterium tuberculosis from Multiple Laboratories

摘要: The publication of the complete genome sequence for Mycobacterium tuberculosis H37Rv in 1998 has had a great impact on research community. Nonetheless, it is suspected that genetic differences have arisen stocks are maintained different laboratories. In order to assess consistency sequences among strains use and extent which they diverged from original strain sequenced, we carried out whole-genome sequencing six Polymorphisms at 73 sites were observed, shared lab strains, though 72 these also with H37Ra likely be due errors reference sequence. An updated should valuable community as well broader microbial addition, several polymorphisms unique individual identified shown consistent known provenance strains. Aside nucleotide substitutions insertion/deletions, multiple IS6110 transposition events supporting theory play significant role plasticity M. genome. This genome-wide catalog can help explain any phenotypic might found, including frameshift mutation mycocerosic acid synthase gene causes two deficient biosynthesis surface glycolipid phthiocerol dimycocerosate (PDIM). resequencing represents fortuitous “in vitro evolution” experiment demonstrates how continues evolve even controlled environment.