Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration.
作者： Jeffrey Bonadio , Elizabeth Smiley , Pravin Patil , Steven Goldstein
DOI: 10.1038/10473
关键词: Cell biology 、 Biology 、 Growth factor 、 Toxicity 、 Gene 、 Plasmid 、 Bone Injury 、 Bone regeneration 、 Gene delivery 、 Molecular biology 、 In vivo
摘要: The inability to deliver growth factors locally in a transient but sustained manner is substantial barrier tissue regeneration. Systems capable of localized plasmid gene delivery for prolonged times may offer lower toxicity and should be well-suited factor therapeutics. We investigated the potency from genes physically entrapped polymer matrix (gene activated matrix) using bone regeneration as endpoint vivo. Implantation matrices at sites injury was associated with retention expression DNA least 6 weeks, induction centimeters normal new stable, reproducible, dose- time-dependent manner.
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