Effect of a Cyclooxygenase‐2 Inhibitor on Interleukin‐1β–Stimulated Activation of the Transcription Factor Nuclear Factor‐Kappa B in Human Gingival Fibroblasts
作者： David A. Tipton , Denise C. Gay , Vaughn A. DeCoster
关键词: Interleukin 6 、 Endocrinology 、 Cyclooxygenase 、 Transcription factor 、 Phosphorylation 、 Prostaglandin E2 、 Protein subunit 、 Fibroblast 、 Interleukin 、 Molecular biology 、 Biology 、 Internal medicine
摘要: Background: In previous work, the cyclooxygenase-2 inhibitor NS-398 inhibited interleukin (IL)-1β–stimulated prostaglandin E2 (PGE2) production almost completely while partially inhibiting IL-6 in aggressive periodontitis (AgP) human gingival fibroblasts. PGE2 and transcription factor nuclear factor-kappa B (NF-κB) regulate IL-1β–stimulated production. Cytoplasmic NF-κB is bound to inhibitors (IκB proteins). IL-1β initiates a cascade resulting phosphorylation degradation of IκB, allowing translocation target gene activation. The purpose this study was determine whether IκB activation.Methods: AgP fibroblasts (1 2 × 106) were exposed 10−11M) with or without (10 nM) serum-free medium. subunit p65 phospho-IκBα measured whole cell, cytoplasmic, extracts, using colorimetric assays. Enzyme-linked immunosorbent assays used measure pr...
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