Enzymatic conversion of bilirubin monoglucuronide to diglucuronide by rat liver plasma membranes.
作者： P L Jansen , J R Chowdhury , E B Fischberg , I M Arias
DOI: 10.1016/S0021-9258(17)40517-5
关键词: Specific activity 、 Biochemistry 、 Microsome 、 Metabolite 、 Bilirubin 、 Glucuronic acid 、 Liver cell 、 Chemistry 、 Bilirubin diglucuronide 、 Glucuronosyltransferase 、 Cell biology 、 Molecular biology
摘要: Formation of bilirubin monoglucuronide from unconjugated requires a microsomal enzyme, UDP-glucuronate glucuronyltransferase (EC 2.4.1.17). Conversion to diglucuronide, the major conjugate in bile, was studied subcellular fractions rat liver. The highest specific activity for diglucuronide formation occurred fraction highly enriched plasma membranes. Studies reaction stoichiometry and utilization UDP-D-[14C]glucuronic acid revealed that conversion is not catalyzed by UDP-glucuronyltransferase, results transglucuronidation monoglucuronide, with bilirubin. When infused intravenously into rats at rates exceeding maximal hepatic excretory capacity, accumulated serum found exclusively bile as predominant metabolite. These suggest occurs surface membrane liver cell. may play role transport glucuronides bile.
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