5-HT2A receptor enhancement of contractile activity of the porcine urothelium and lamina propria

摘要: Objectives To examine the effect of 5-hydroxytryptamine (5-HT; serotonin) on contractile properties urothelium and lamina propria, as a better understanding bladder physiology might aid development new treatments. Methods Strips porcine propria were suspended in gassed Krebs-bicarbonate solution, cumulative concentration–response curves for 5-HT generated absence presence antagonists, Nω-nitro-l-arginine indomethacin. Responses to α-methyl-5-HT also examined. Results Strips urothelium/lamina developed spontaneous contractions, whereas addition induced concentration-dependent increases tone with maximal contractions 50.43 ± 2.78 mN/g tissue weight (n = 100). Tonic unchanged (100 μmol/L) or indomethacin (5 μmol/L). Selective concentrations antagonists methiothepin (5-HT1&2, 100 nmol/L), RS102221 (5-HT2C, 30 ondansetron (5-HT3, GR113808, (5-HT4, SB699551 (5-HT5, 10 SB399885 (5-HT6, nmol/L) SB269970 (5-HT7, did not influence responses 5-HT. However, 5-HT2A antagonist, ketanserin (30–300 μmol/L), caused shift curve yielding an affinity estimate 7.9. Conclusions The results show that are predominantly mediated through receptor.